Mouse Cardiac Tn-I (High Sensitivity) Elisa Kit

  • Antigen information

Access number: P19429

Gene ID: 7137

Genetic symbols: TNNC1, TNNI3

Protein name and synonyms:

troponin I, heart muscle (cardiac troponin I)

Target species: Human

  • Test format

Detection range:

0.38 pg / ml – 625 pg / ml

Recommended dilution (serum and plasma): 2 times

Sensitivity: 0.38 pg / ml

Specificity:

This ELISA antibody pair detects human cTnI. Other species not determined.

Number of targets detected: 1

Species detected: Human

Compatible sample types:

Cell culture supernatants Plasma Serum

Design Principle:

Sandwich-based

Detection method: colourimetric

Quantitative / semi-quantitative:

Quantitative

Solid support: 96-well microplate

  • Product specifications

Size: 1, 2, or 5 x 96-Well Strip Microplate Kit

  • Product characteristics

Additional notes

  1. Strip plates and additional reagents allow for use in multiple experiments
  2. Quantitative protein detection
  3. Set normal range
  4. Best Products for Antibody Array Data Confirmation
  • Application notes

Kit components

  1. Precoated 96-Well Strip Microplate
  2. Wash buffer
  3. Stop solution
  4. Test diluent (s)
  5. Lyophilized standard
  6. Biotinylated detection antibody
  7. Streptavidin-conjugated HRP
  8. Single-pass substrate TMB

Other Necessary Materials

  • Distilled or deionized water
  • Precision pipettes to deliver volumes from 2 µl to 1 µl
  • Adjustable 1-25 µl pipettes for reagent preparation
  • Graduated cylinders of 100 µl and 1 liter
  • Tubes for preparing standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450 nm
  • Log-log graph paper or computer and software for ELISA data analysis

Protocol outline

  1. Prepare all reagents, samples, and standards as directed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O / N at 4 ° C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage stability

The complete kit can be stored at -20 ° C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit can be stored at 4 ° C for up to 6 months. For long-term storage, it is recommended to store at -80 ° C.

Use

  • This product is provided for LABORATORY RESEARCH USE ONLY.
  • It is not for diagnostic or therapeutic use.

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