Recombinant Cyno NKG2a & CD94 Heterodimer

Product specifications

Purity

>95%, by SDS-PAGE, visualized with silver staining and quantitative densitometry by Coomassie® blue staining.

Endotoxin level

<0.10 EU per 1 μg of protein by the LAL method.

Activity

Measured by its binding capacity in a functional ELISA. When recombinant mouse Fc chimaera NKG2A / CD159a is coated at 0.5 µg / ml, recombinant human CD94 binds with a typical ED50 of 0.1-0.6 μg / ml.

Access No: Q9Z202

Structure / Form: disulfide-bonded homodimer

Predicted molecular mass: 44 kDa

SDS-PAGE: 58-67 kDa, reducing conditions

What does CF mean?

CF stands for Carrier Free (CF). We generally add bovine serum albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein improves the stability of the protein, increases shelf life, and allows the recombinant protein to be stored in a more dilute concentration. The free version of the operator does not contain BSA.

Which formulation is right for me?

In general, we recommend purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, carrier-free protein is recommended for applications where the presence of BSA could interfere.

Formulation:

Lyophilized from a 0.2 µm filtered solution in PBS.

Reconstitution

Reconstitute at 500 μg / mL in PBS.

Shipping

The product is shipped at room temperature. Upon receipt, please store immediately at the recommended temperature below.

Stability and Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 ° C as supplied.
  • 1 month at 2 to 8 ° C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 ° C under sterile conditions after reconstitution.

Background: NKG2A / CD159a

NKG2A / CD159a is an approximately 40 kDa transmembrane C-type lectin superfamily protein that inhibits the activation of the innate immune system (1). The NKG2A mouse consists of a 70 amino acid (aa) cytoplasmic domain with two ITIM inhibitory motifs, a 23 aa transmembrane segment, and a 151 aa extracellular domain (ECD) with a C-type lectin domain (2). Within the ECD, mouse NKG2A shares a 41% and 71% sequence identity with human and rat NKG2A, respectively.

Alternative splicing generates additional isoforms with a 17 aa deletion in the extracellular juxtamembrane region or substitution of that region plus the transmembrane segment. NKG2A is expressed in a subset of NK cells and CD8 + T cells (2-6) where it forms a heterodimer covalent with CD94 (5, 7, 8). NKG2A-CD94 heterodimers bind to the widely expressed non-classical MHC-I molecule, HLA-E (Qa-1b in mouse), which has a peptide derived from the signal peptide of classical MHC-I molecules (2, 7). Activation of the NKG2A-CD94 complex inhibits the cytolytic activity of NK and CD8 + T cells (2, 3, 5, 6, 9).

This allows the innate immune system to detect cells that express host MHC-I molecules and protect them from NK cell-mediated lysis. This mechanism is altered by the human cytomegalovirus which encodes a peptide that is homologous to the HLA-E binding peptide (10). HCMV-infected cells positively regulate the expression of HLA-E and NKG2A and use this peptide to escape immune clearance (3, 10). In contrast, the vaccinia virus induces HLA-E down-regulation, allowing the lysis of NK cells from the virus-infected cell (11).

Long-named: G2A natural killer

Entrez Gene IDs

3821 (human); 16641 (mouse); 102121106 (Cynomolgus Monkey)

Alternative Names

Member A of the CD159-like antigen family; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1 / B activating NK receptor; NKG2A; NKG2-A; NKG2-A / B type II integral membrane protein; NKG2-A / B activating NK receptor; NKG2-A / NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B

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